An In Vitro Study on Minimal Inhibitory Concentration of Combinations of Extra Virgin Olive Oil, Curcuma zedoaria and Azadirachta indica on Oral Microbes Streptococcus mutans and Candida albicans
Anshula N Deshpande, Simron Baishya, Khushali Shah, Vandana S Shah, Sneha Dori
Citation Information :
Deshpande A N, Baishya S, Shah K, Shah VS, Dori S. An In Vitro Study on Minimal Inhibitory Concentration of Combinations of Extra Virgin Olive Oil, Curcuma zedoaria and Azadirachta indica on Oral Microbes Streptococcus mutans and Candida albicans. J South Asian Assoc Pediatr Dent 2024; 7 (3):156-161.
Introduction: Extra virgin olive oil (EVOO), Curcuma zedoaria, and Azadirachta indica are all known to be antimicrobial. Herbal products used in dentistry, such as EVOO, Curcuma (turmeric), and A. indica (neem), have grown in popularity due to their antibacterial, anti-inflammatory, and antioxidant characteristics, which aid in the prevention and treatment of oral diseases. The synergistic action of these oils has the potential to change dental care by efficiently targeting harmful microorganisms, such as Streptococcus mutans and Candida albicans.
Aim: The aim of the present study was to determine minimal inhibitory concentrations (MICs) of EVOO with C. zedoaria (OC), EVOO with A. indica (OA), and EVOO with A. indica and C. zedoaria (OAC) on S. mutans and C. albicans.
Materials and methods: The oil preparation was done by combining 65–90% EVOO with 10–35% C. zedoaria, 70–95% EVOO with 5–30% A. indica, and 50% EVOO with 20% A. indica and 30% C. zedoaria vol/vol, respectively, by use of an insulin syringe. After the procuring of S. mutans and C. albicans, MIC was determined using the broth dilution method.
Results: The combinations of 70% EVOO + 30% C. zedoaria (2.5 µg/mL), 65% EVOO + 35% C. zedoaria (4.5 µg/mL), 80% EVOO + 20% A. indica (3 µg/mL), 75% EVOO + 25% A. indica (3.5 µg/mL), and 70% EVOO + 30% A. indica (4.5 µg/mL) effectively inhibited the growth of S. mutans. Similarly, 75% EVOO + 25% C. zedoaria (3.5 µg/mL), 70% EVOO + 30% C. zedoaria (4 µg/mL), 65% EVOO + 35% C. zedoaria (4.5 µg/mL), 80% EVOO + 20% A. indica (4 µg/mL), 75% EVOO + 25% A. indica (4.5 µg/mL), and 70% EVOO + 30% A. indica (6 µg/mL) inhibited C. albicans. However, no growth inhibition for either S. mutans or C. albicans was observed with the OAC combination.
Conclusion: The MIC for S. mutans in the OC group is 70% EVOO + 30% C. zedoaria, and for C. albicans is 75% EVOO + 25% C. zedoaria. The MIC for both S. mutans and C. albicans in the OA group is 80% EVOO + 20% A. indica. The synergistic effect of 65% EVOO with 35% C. zedoaria and 70% EVOO with 30% A. indica inhibited the highest microbial growth for both S. mutans and C. albicans.
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