Journal of South Asian Association of Pediatric Dentistry

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VOLUME 6 , ISSUE 3 ( September-December, 2023 ) > List of Articles


Comparative Assessment of the Association between Salivary Vitamin D Levels and Early Childhood Caries Using Enzyme-linked Immunosorbent Assay

Umme Azher, Aishwarya Chidambareshwar

Keywords : Biofluid, Dental decay, Early childhood caries, Saliva, Vitamin D

Citation Information : Azher U, Chidambareshwar A. Comparative Assessment of the Association between Salivary Vitamin D Levels and Early Childhood Caries Using Enzyme-linked Immunosorbent Assay. J South Asian Assoc Pediatr Dent 2023; 6 (3):114-117.

DOI: 10.5005/jp-journals-10077-3284

License: CC BY-NC 4.0

Published Online: 30-12-2023

Copyright Statement:  Copyright © 2023; The Author(s).


Aim: Evaluation of interrelation between vitamin D levels in saliva and early childhood caries (ECC) utilizing enzyme-linked immunosorbent assay (ELISA). Materials and methods: The sample selection in the cross-sectional, case-control study involved—(1) an interview with the parents/caregivers through a questionnaire; (2) a clinical examination of children to ascertain dental decay status; those with severe ECC (S-ECC) were enlisted in group I (n = 15) and children with a decayed, missing, and filled surfaces (dmfs) score of 0 to group II (n = 15). The samples of unstimulated whole saliva (5 mL) were collected, and the salivary vitamin D levels were measured using a human 25-dihydroxy vitamin D3 (25-OHD) ELISA kit. Results: Subjects in groups I and II showed mean salivary vitamin D levels of 8.8 + 4.92 and 16.8 + 10.8 ng/mL, respectively. The vitamin D levels in saliva exhibited a difference statistically. Conclusion: Variations in vitamin D scores in the saliva of children with S-ECC and those caries-free demonstrate that vitamin D in saliva can serve as a marker of caries risk in children. Clinical significance: The simplicity and noninvasiveness of saliva collection, especially in children, confers the advantage of a more acceptable and cost-effective biofluid for caries-risk factors assessment.

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