Journal of South Asian Association of Pediatric Dentistry

Register      Login

VOLUME 6 , ISSUE 1 ( January-April, 2023 ) > List of Articles

ORIGINAL RESEARCH

Comparative Evaluation of Influence of Self-assembling Peptide P11-4 Based Remineralization Agent on Incipient Dental Caries Lesions of Primary Teeth: A Polarized Light Microscopic Study

Zaina Gayas, Umme Azher, Santhosh T Paul, Divya Reddy

Keywords : Dental caries, Fluoride, Remineralization, Self-assembling peptide, White spot lesion

Citation Information : Gayas Z, Azher U, Paul ST, Reddy D. Comparative Evaluation of Influence of Self-assembling Peptide P11-4 Based Remineralization Agent on Incipient Dental Caries Lesions of Primary Teeth: A Polarized Light Microscopic Study. J South Asian Assoc Pediatr Dent 2023; 6 (1):3-8.

DOI: 10.5005/jp-journals-10077-3250

License: CC BY-NC 4.0

Published Online: 22-04-2023

Copyright Statement:  Copyright © 2023; The Author(s).


Abstract

Background: Interception of the progression of incipient dental caries lesions with remineralization agents can facilitate their regression noninvasively. Aim: To evaluate the influence of self-assembling peptide P11-4 (oligopeptide 104) based remineralization agent in comparison to fluoride-enhanced hydroxyapatite gel on incipient caries lesions in primary teeth. Materials and methods: A total of 36 sound deciduous molars were selected and coated with two layers of acid-resistant varnish, leaving two windows (2 × 2 mm) on the buccal surface. The teeth were immersed in the demineralizing solution and incubated at 37°C for 4 days to produce artificial enamel caries lesions. One of the windows was assigned as a baseline lesion, while the other was designated as an experimental lesion and exposed to the test product and pH cycling. The samples were divided into three groups (n = 12). Group I—self-assembling peptide P11-4 gel, group II—fluoride-enhanced hydroxyapatite gel, and group III—control. The self-assembling peptide P11-4 and fluoride-enhanced hydroxyapatite were applied to the specimens for 5–3 minutes, respectively. The specimens were subjected to a 7-day pH-cycling regimen, followed by sectioning and examination under a polarized light microscope. Results: The remineralization agents demonstrated a decrease in mean lesion depth in comparison to baseline, with the group I demonstrating greater reduction (165.235 ± 7.569–107.704 ± 6.735 μm) in comparison to group II (175.365 ± 12.238–127.462 ± 7.111 μm). Conclusion: The self-assembling peptide P11-4 and fluoride-enhanced hydroxyapatite gel exhibited significant potential in the remineralization of artificial enamel caries lesions in primary teeth. However, the self-assembling peptide P11-4 demonstrated greater remineralization potential.


HTML PDF Share
  1. Kind L, Stevanovic S, Wuttig S, et al. Biomimetic remineralization of carious lesions by self-assembling peptide. J Dent Res 2017;96(7):790–797. DOI: 10.1177/0022034517698419
  2. Featherstone JDB. Remineralization, the natural caries repair process–the need for new approaches. Adv Dent Res 2009;21(1):4–7. DOI: 10.1177/0895937409335590
  3. Prabhakar AR, Arali V. Comparison of the remineralizing effects of sodium fluoride and bioactive glass using bioerodible gel systems. J Dent Res Dent Clin Dent Prospects 2009;3(4):117–121. DOI: 10.5681/joddd.2009.029
  4. Kamal D, Hassanein H, Elkassas D, et al. Comparative evaluation of remineralizing efficacy of biomimetic self-assembling peptide on artificially induced enamel lesions: an in vitro study. J Conserv Dent 2018;21(5):536–541. DOI: 10.4103/JCD.JCD_123_18
  5. Soares R, Fernandes M, Lambor R. Assessment of enamel remineralization after treatment with four different remineralising agents: a scanning electron microscopy (SEM) study. J Clin Diagn Res 2017;11(4):ZC136–ZC141. DOI: 10.7860/JCDR/2017/23594.97882017
  6. Sindhura V, Uloopi KS, Vinay C, et al. Evaluation of enamel remineralizing potential of self-assembling peptide P11-4 on artificially induced enamel lesions in vitro. J Indian Soc Pedod Prev Dent 2018;36(4):352–356. DOI: 10.4103/JISPPD.JISPPD_255_18
  7. Alkilzy M, Santamaria RM, Schmoeckel J, et al. Treatment of carious lesions using self-assembling peptides. Adv Dent Res 2018;29(1):42–47. DOI: 10.1177/0022034517737025
  8. Asano K, Kawamoto R, Iino M, et al. Effect of pre-reacted glass-ionomer filler extraction solution on demineralization of bovine enamel. Oper Dent 2014;39(2):159–165. DOI: 10.2341/13-034-L
  9. González-Cabezas C. The chemistry of caries: remineralization and demineralization events with direct clinical relevance. Dent Clin North Am 2010;54(3):469–478. DOI: 10.1016/j.cden.2010.03.004
  10. Brunton PA, Davies RP, Burke JL, et al. Treatment of early caries lesions using biomimetic self-assembling peptides–a clinical safety trial. Br Dent J 2013;215(4):E6. DOI: 10.1038/sj.bdj.2013.741
  11. Kirkham J, Firth A, Vernals D, et al. Self-assembling peptide scaffolds promote enamel remineralization. J Dent Res 2007;86(5):426–430. DOI: 10.1177/154405910708600507
  12. Featherstone JD, Shariati M, Arends J, et al. Comparison of artificial caries-like lesions by quantitative microradiography and microhardness profiles. Caries Res 1983;17(5):385–391. DOI: 10.1159/000260692
  13. Hicks MJ, Flaitz CM. Enamel caries formation and lesion progression with a fluoride dentifrice and a calcium-phosphate containing fluoride dentifrice: a polarized light microscopic study. ASDC J Dent Child 2000;67(1):21–28.
  14. Magalhães AC, Moron BM, Comar LP, et al. Comparison of cross-sectional hardness and transverse microradiography of artificial carious enamel lesions induced by different demineralising solutions and gels. Caries Res 2009;43(6):474–483. DOI: 10.1159/000264685
  15. Buzalaf MAR, Hannas AR, Magalhães AC, et al. pH-cycling models for in vitro evaluation of the efficacy of fluoridated dentifrices for caries control: strengths and limitations. J Appl Oral Sci 2010;18(4):316–334. DOI: 10.1590/s1678-77572010000400002
  16. Whittaker DK. Structural variations in the surface zone of human tooth enamel observed by scanning electron microscopy. Arch Oral Biol 1982;27(5):383–392. DOI: 10.1016/0003-9969(82)90147-9
  17. Yetkiner E, Eden E, Attin R, et al. Comparative evaluation of fluoride varnishes, self-assembling peptide-based remineralization agent, and enamel matrix protein derivative on artificial enamel remineralization in vitro. Prog Orthod 2021;22(1):4. DOI: 10.1186/s40510-020-00345
  18. Takahashi F, Kurokawa H, Shibasaki S, et al. Ultrasonic assessment of the effects of self-assembling peptide scaffolds on preventing enamel demineralization. Acta Odontol Scand 2016;74(2):142–147. DOI: 10.3109/00016357.2015.1066850
  19. Silvertown JD, Wong BPY, Sivagurunathan KS, et al. Remineralization of natural early caries lesions in vitro by P11-4 monitored with photothermal radiometry and luminescence. J Investig Clin Dent 2017;8(4):e12257. DOI: 10.1111/jicd.12257
  20. Schmidlin P, Zobrist K, Attin T, et al. In vitro re-hardening of artificial enamel caries lesions using enamel matrix proteins or self-assembling peptides. J Appl Oral Sci 2016;24(1):31–36. DOI: 10.1590/1678-775720150352
  21. Jablonski-Momeni A, Heinzel-Gutenbrunner M. Efficacy of the self-assembling peptide P11-4 in constructing a remineralization scaffold on artificially-induced enamel lesions on smooth surfaces. J Orofac Orthop 2014;75(3):175–190. DOI: 10.1007/s00056-014-0211-2
  22. Wierichs RJ, Kogel J, Lausch J, et al. Effects of self-assembling peptide P11-4, fluorides and caries infiltration on artificial enamel caries lesions in vitro. Caries Res 2017;51(5):451–459. DOI: 10.1159/000477215
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.