Context: The concept of the revascularization technique mainly depends on root canal sterilization, the presence of a template (blood clot), and coronary filling, which prevents marginal leakage. Successful disinfection is carried out by using triple antibiotic paste (TAP) as an intracanal medication due to its excellent antimicrobial activity, but its use is associated with discoloration of the tooth crown, the development of bacterial resistance, and allergic reactions. Aims: To evaluate the antibacterial efficacy of triple organic paste vs TAP against strains of Enterococcus faecalis, Streptococcus mutans, and Candida albicans. Methods and material: Natural products were divided into six groups and further divided into 18 subgroups. Minimum inhibitory concentration (MIC) was determined for each group using the trial and error method, and results were obtained after incubation for 24 hours at 37°C. Antibacterial efficacy was determined by zone of inhibition and compared with TAP (control group) individually against E. faecalis, S. mutans, and C. albicans. The values were recorded and subjected to statistical analysis by one-way analysis of variance (ANOVA) and Tukey's post hoc test. Results: All the subgroups have shown significant zone of inhibition values against E. faecalis, S. mutans, and C. albicans, except for the subgroup IC, where no statistically significant values were found when compared with the control group. Conclusion: Most of the combinations of natural products have shown better antibacterial efficacy when compared with TAP, suggesting its use to overcome the disadvantages of TAP. Further clinical studies are required for evaluating the antibacterial efficacy in vivo.
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