Journal of South Asian Association of Pediatric Dentistry

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VOLUME 5 , ISSUE 3 ( September-December, 2022 ) > List of Articles


Prevalence of Streptococcus mutans and Candida dubliniensis in Plaque of Caries-free and Caries-active 3–6-year-old Children by Using Polymerase Chain Reaction: A Clinical Study

Poornima Parameshwarappa, Yanina Singh, Mebin G Mathew, Mallikarjuna Kenchappa, Nagaveni Nandanhosur Basavanthappa, Roopa Korishettar

Keywords : Candida dubliniensis, Dental caries, Polymerase chain reaction, Streptococcus mutans

Citation Information : Parameshwarappa P, Singh Y, Mathew MG, Kenchappa M, Basavanthappa NN, Korishettar R. Prevalence of Streptococcus mutans and Candida dubliniensis in Plaque of Caries-free and Caries-active 3–6-year-old Children by Using Polymerase Chain Reaction: A Clinical Study. J South Asian Assoc Pediatr Dent 2022; 5 (3):132-135.

DOI: 10.5005/jp-journals-10077-3238

License: CC BY-NC 4.0

Published Online: 27-12-2022

Copyright Statement:  Copyright © 2022; The Author(s).


Introduction: Oral cavity harbors numerous types of microbial flora, which change frequently with changes in the environment and which in turn leads to the process of caries. Aims: To evaluate the prevalence of Streptococus mutans (S. mutans) and Candida dubliniensis (C. dubliniensis) in dental plaque of caries-free (CF) and carries-active (CA) children aged 3–6 years using polymerase chain reaction (PCR). Material and methods: A total of 18 CA and CF children in the age group of 3–6 years were randomly selected. Plaque samples were collected using sterile micro brushes from teeth. Specific primers were used to carry out PCR in the plaque samples. Statistical analysis was done using Fisher's exact test. Results: In CA group, S. mutans were seen in 61.1% of children which is statistically significant with a p-value of 0.04 and C. dubliniensis in 27.8% of children whereas, in CF group, S. mutans were present in 27.8% of participants and C. dubliniensis in 5.6%. These results show that both organisms were more predominant in the CA group. Conclusion: Though both S. mutans (61.1%) and C. dubliniensis (27.8%) were present in CA group, S. mutans was strongly associated with dental caries. In the individuals with high scores of def and international caries detection and assessment system II, both species were more prevalent.

  1. Pitts NB, Zero DT, Marsh PD, et al. Dental caries. Nat Rev Dis Primers 2017;3:17030. DOI: 10.1038/nrdp.2017.30
  2. Dye BA, Arevalo O, Vargas CM. Trends in paediatric dental caries by poverty status in the United States, 1988-1994 and 1999-2004. Int J Paediatr Dent 2010;20(2):132–143. DOI: 10.1111/j.1365-263X.2009.01029.x
  3. Valdez RMA, Duque C, Caiaffa KS, et al. Genotypic diversity and phenotypic traits of Streptococcus mutans isolates and their relation to severity of early childhood caries. BMC Oral Health 2017;17(1):115. DOI: 10.1186/s12903-017-0406-1
  4. Klinke T, Guggenheim B, Klimm W, et al. Dental caries in rats associated with Candida albicans. Caries Res 2011;45(2):100–106. DOI: 10.1159/000324809
  5. Okada M, Kawamura M, Oda Y, et al. Caries prevalence associated with Streptococcus mutans and Streptococcus sobrinus in Japanese schoolchildren. Int J Paediatr Dent 2012;22(5):342–348. DOI: 10.1111/j.1365-263X.2011.01203.x
  6. de Carvalho FG, Silva DS, Hebling J, et al. Presence of mutans streptococci and Candida spp. in dental plaque/dentine of carious teeth and early childhood caries. Arch Oral Biol 2006;51(11):1024–1028. DOI: 10.1016/j.archoralbio.2006.06.001
  7. Al-Ahmad A, Auschill TM, Dakhel R, et al. Prevalence of Candida albicans and Candida dubliniensis in caries-free and caries-active children in relation to the oral microbiota-a clinical study. Clin Oral Investig 2016;20(8):1963–1971. DOI: 10.1007/s00784-015-1696-9
  8. Alnuaimi AD, O'Brien-Simpson NM, Reynolds EC, et al. Clinical isolates and laboratory reference Candida species and strains have varying abilities to form biofilms. FEMS Yeast Res 2013;13(7):689–699. DOI: 10.1111/1567-1364.12068
  9. Henriques M, Azeredo J, Oliveira R. Candida albicans and Candida dubliniensis: comparison of biofilm formation in terms of biomass and activity. Br J Biomed Sci 2006;63(1):5–11. DOI: 10.1080/09674845.2006.11732712
  10. Villhauer AL, Lynch DJ, Drake DR. Improved method for rapid and accurate isolation and identification of Streptococcus mutans and Streptococcus sobrinus from human plaque samples. J Microbiol Methods 2017;139:205–209. DOI: 10.1016/j.mimet.2017.06.009
  11. Damle SG, Loomba A, Dhindsa A, et al. Correlation between dental caries experience and mutans streptococci counts by microbial and molecular (polymerase chain reaction) assay using saliva as microbial risk indicator. Dent Res J (Isfahan) 2016;13(6):552–559. DOI: 10.4103/1735-3327.197035
  12. Fragkou S, Balasouli C, Tsuzukibashi O, et al. Streptococcus mutans, Streptococcus sobrinus and Candida albicans in oral samples from caries-free and caries-active children. Eur Arch Paediatr Dent 2016;17(5):367-375. DOI: 10.1007/s40368-016-0239-7
  13. Fujiwara T, Sasada E, Mima N, et al. Caries prevalence and salivary mutans streptococci in 0–2-year-old children of Japan. Community Dent Oral Epidemiol 1991;19(3):151–154. DOI: 10.1111/j.1600-0528.1991.tb00131.x
  14. Hata S, Hata H, Miyasawa-Hori H, et al. Quantitative detection of Streptococcus mutans in the dental plaque of Japanese preschool children by real-time PCR. Lett Appl Microbiol 2006;42(2):127–131. DOI: 10.1111/j.1472-765X.2005.01821.x
  15. Vacharaksa A, Suvansopee P, Opaswanich N, et al. PCR detection of Scardovia wiggsiae in combination with Streptococcus mutans for early childhood caries-risk prediction. Eur J Oral Sci 2015;123(5):312–318. DOI: 10.1111/eos.12208
  16. Loyola-Rodriguez JP, Martinez-Martinez RE, Flores-Ferreyra BI, et al. Distribution of Streptococcus mutans and Streptococcus sobrinus in saliva of Mexican preschool caries-free and caries-active children by microbial and molecular (PCR) assays. J Clin Pediatr Dent 2008;32(2):121–126. DOI: 10.17796/jcpd.32.2.cm00062530v856r4
  17. Matee MI, Mikx FH, Maselle SY, et al. Mutans streptococci and lactobacilli in breast-fed children with rampant caries. Caries Res 1992;26(3):183–187. DOI: 10.1159/000261440
  18. Kneist S, Borutta A, Sigusch BW, et al. First-time isolation of Candida dubliniensis from plaque and carious dentine of primary teeth. Eur Arch Paediatr Dent 2015;16(4):365–370. DOI: 10.1007/s40368-015-0180-1
  19. Lozano Moraga CP, Rodríguez Martínez GA, Lefimil Puente CA, et al. Prevalence of Candida albicans and carriage of Candida non-albicans in the saliva of preschool children, according to their caries status. Acta Odontol Scand 2017;75(1):30–35. DOI: 10.1080/00016357.2016.1244560
  20. de Jesus VC, Shikder R, Oryniak D, et al. Sex-based diverse plaque microbiota in children with severe caries. J Dent Res 2020;99:703–712. DOI: 10.1177/0022034520908595
  21. Garibyan L, Avashia N. Polymerase chain reaction. J Invest Dermatol 2013;133(3):1–4. DOI: 10.1038/jid.2013.1
  22. Milgrom P, Riedy CA, Weinstein P, et al. Dental caries and its relationship to bacterial infection, hypoplasia, diet, and oral hygiene in 6- to 36-month-old children. Community Dent Oral Epidemiol 2000;28(4):295–306. DOI: 10.1034/j.1600-0528.2000.280408.x
  23. Karn TA, O'Sullivan DM, Tinanoff N. Colonization of mutans streptococci in 8- to 15-month-old children. J Public Health Dent 1998;58(3):248–249. DOI: 10.1111/j.1752-7325.1998.tb03001.x
  24. Ghazal TS, Levy SM, Childers NK, et al. Mutans streptococci and dental caries: a new statistical modeling approach. Caries Res 2018;52(3):246–252. DOI: 10.1159/000486103
  25. Menon LU, Scoffield JA, Jackson JG, et al. Candida albicans and early childhood caries. Front Dent Med 2022;(3):849274. DOI: 10.3389/fdmed.2022.8492
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