Journal of South Asian Association of Pediatric Dentistry

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VOLUME 5 , ISSUE 3 ( September-December, 2022 ) > List of Articles

ORIGINAL RESEARCH

Prevalence of Streptococcus mutans and Candida dubliniensis in Plaque of Caries-free and Caries-active 3–6-year-old Children by Using Polymerase Chain Reaction: A Clinical Study

Poornima Parameshwarappa, Yanina Singh, Mebin G Mathew, Mallikarjuna Kenchappa, Nagaveni Nandanhosur Basavanthappa, Roopa Korishettar

Keywords : Candida dubliniensis, Dental caries, Polymerase chain reaction, Streptococcus mutans

Citation Information : Parameshwarappa P, Singh Y, Mathew MG, Kenchappa M, Basavanthappa NN, Korishettar R. Prevalence of Streptococcus mutans and Candida dubliniensis in Plaque of Caries-free and Caries-active 3–6-year-old Children by Using Polymerase Chain Reaction: A Clinical Study. J South Asian Assoc Pediatr Dent 2022; 5 (3):132-135.

DOI: 10.5005/jp-journals-10077-3238

License: CC BY-NC 4.0

Published Online: 27-12-2022

Copyright Statement:  Copyright © 2022; The Author(s).


Abstract

Introduction: Oral cavity harbors numerous types of microbial flora, which change frequently with changes in the environment and which in turn leads to the process of caries. Aims: To evaluate the prevalence of Streptococus mutans (S. mutans) and Candida dubliniensis (C. dubliniensis) in dental plaque of caries-free (CF) and carries-active (CA) children aged 3–6 years using polymerase chain reaction (PCR). Material and methods: A total of 18 CA and CF children in the age group of 3–6 years were randomly selected. Plaque samples were collected using sterile micro brushes from teeth. Specific primers were used to carry out PCR in the plaque samples. Statistical analysis was done using Fisher's exact test. Results: In CA group, S. mutans were seen in 61.1% of children which is statistically significant with a p-value of 0.04 and C. dubliniensis in 27.8% of children whereas, in CF group, S. mutans were present in 27.8% of participants and C. dubliniensis in 5.6%. These results show that both organisms were more predominant in the CA group. Conclusion: Though both S. mutans (61.1%) and C. dubliniensis (27.8%) were present in CA group, S. mutans was strongly associated with dental caries. In the individuals with high scores of def and international caries detection and assessment system II, both species were more prevalent.

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