Journal of South Asian Association of Pediatric Dentistry

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VOLUME 5 , ISSUE 1 ( January-April, 2022 ) > List of Articles

ORIGINAL RESEARCH

Antibacterial Activity of Garlic Extract against Streptococcus mutans and Lactobacillus acidophilus: An In Vitro Study

Raghu Devaraju, K Arulselvan, Zaina Gayas, Vishnu Uday

Keywords : Allium sativum, Antimicrobial activity, Formocresol, L. acidophilus, S. mutans

Citation Information : Devaraju R, Arulselvan K, Gayas Z, Uday V. Antibacterial Activity of Garlic Extract against Streptococcus mutans and Lactobacillus acidophilus: An In Vitro Study. J South Asian Assoc Pediatr Dent 2022; 5 (1):26-31.

DOI: 10.5005/jp-journals-10077-3218

License: CC BY-NC 4.0

Published Online: 29-03-2022

Copyright Statement:  Copyright © 2022; The Author(s).


Abstract

Background: Pulpotomy is a therapeutic conservative procedure involving the amputation of the infected coronal pulp and dressing the remaining noninfected pulp tissue with a suitable material. An antimicrobial pulp dressing material is key to avoid failure of the treatment due to any residual microflora. Allium sativum, commonly known as “Garlic” is one of the most widely researched natural entities due to its antimicrobial, antioxidant, and antifungal properties. The aim of the present study was to evaluate the antimicrobial efficacy of garlic extract (A. sativum) in the concentrations of 100%, 50%, and 25% against Streptococcus mutans and Lactobacillus acidophilus using the agar well diffusion method. Methodology: The microorganisms S. mutans and L. acidophilus were isolated from the patient\'s saliva and were identified using the biochemical test. Five wells were prepared using a sterile template in agar plates. The garlic extract in the concentrations of 100%, 50%, and 25%, formocresol (positive control), and ethanol (negative control) was added to the prepared wells. The agar plates were incubated at 37°C for 24 hours and 48 hours in a candle extinction jar for S. mutans and an anaerobic jar for L. acidophilus, respectively. Results: All the tested concentrations of the garlic extract demonstrated antimicrobial activity against S. mutans and L. acidophilus. Formocresol in 1:5 concentration (positive control) showed excellent antimicrobial activity and no antimicrobial activity was observed with ethanol (negative control) at 24 hours and 48 hours. Antimicrobial activity is reduced by reducing the concentration of garlic extract. It was found that L. acidophilus was more sensitive to different concentrations of garlic extract than S. mutans. Conclusion: All the tested concentrations of garlic extracts exhibited varying antimicrobial activities against S. mutans and L. acidophilus. Considering the antimicrobial activity, A. sativum can be considered as a suitable alternative to formocresol as a pulpotomy medicament.


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  1. Bhayat A, Hifnawy T, Mahrous M, et al. Correlating dental caries with oral bacteria and the buffering capacity of saliva in children in Madinah, Saudi Arabia. J Int Soc Prev Community Dent 2013;3(1):38-43. DOI: 10.4103/2231-0762.115712
  2. Yang F, Zhang Y, Yuan X, et al. Caries experience and its association with weight status among 8-year-old children in Qingdao, China. J Int Soc Prev Community Dent 2015;5(1):52–58. DOI: 10.4103/2231-0762.151978
  3. Primosch RE, Glomb TA, Jerrell RG. Primary tooth pulp therapy as taught in predoctoral pediatric dental programs in the United States. Pediatr Dent 1997;19(2):118–122. PMID: 9106874.
  4. Waterhouse PJ, Nunn JH, Whitworth JM. An investigation of the relative efficacy of Buckley's formocresol and calcium hydroxide in primary molar vital pulp therapy. Br Dent J 2000;188(1):32–36. DOI: 10.1038/sj.bdj.4800380
  5. Garcia-Godoy F. A 42 month clinical evaluation of glutaraldehyde pulpotomies in primary teeth. J Pedod 1986;10(2):148–155. PMID: 3086533.
  6. Fuks AB, Eidelman E, Cleaton-Jones P, et al. Pulp response to ferric sulfate, diluted formocresol and IRM in pulpotomized primary baboon teeth. ASDC J Dent Child 1997;64(4):254–259. PMID: 9328676.
  7. Kakarla P, Avula JS, Mellela GM, et al. Dental pulp response to collagen and pulpotec cement as pulpotomy agents in primary dentition: a histological study. J Conserv Dent 2013;16(5):434–438. DOI: 10.4103/0972-0707.117525
  8. Bossù M, Iaculli F, Giorgio GD, et al. Different pulp dressing materials for the pulpotomy of primary teeth: a systematic review of the literature. J Clin Med 2020;9(3):838. DOI: 10.3390/jcm9030838
  9. Schwartz RS, Mauger M, Clement DJ, et al. Mineral trioxide aggregate: a new material for endodontics. J Am Dent Assoc 1999;130(7):967–975. DOI: 10.14219/jada.archive.1999.0337
  10. Junior ES, Santos MGCD, Oliveira LB, et al. MTA and biodentine for primary teeth pulpotomy: a systematic review and meta-analysis of clinical trials. Clin Oral Investig 2019;23(4):1967–1976. DOI: 10.1007/s00784-018-2616-6
  11. Çelik BN, Mutluay MS, Arıkan V, et al. The evaluation of MTA and biodentine as a pulpotomy materials for carious exposures in primary teeth. Clin Oral Investig 2019;23(2):661–666. DOI: 10.1007/s00784-018-2472-4
  12. Schmitt D, Lee J, Bogen G. Multifaceted use of ProRoot MTA root canal repair material. Pediatr Dent 2001;23(4):326–330. PMID: 28292337.
  13. Coll JA, Seale NS, Vargas K, et al. Primary tooth vital pulp therapy: a systematic review and meta-analysis. Pediatr Dent 2017;39(1):16–123. PMID: 28292337.
  14. Mohammad SG, Raheel SA, Baroudi K. Clinical and radiographic evaluation of allium sativum oil as a new medicament for vital pulp treatment of primary teeth. J Int Oral Health 2014;6(6):35–36. PMID: 25628480; PMCID: PMC4295451
  15. Tsao SM, Yin MC. In vitro antimicrobial activity of four diallyl sulphides occurring naturally in garlic and Chinese leek oils. J Med Microbiol 2001;50:646–649. DOI: 10.1099/0022-1317-50-7-646
  16. Narendhirakannan RT, Rajeswari K. In vitro antioxidant properties of three varieties of allium sativum extracts. J Chem 2010;7:S573–S579. DOI: 10.1155/2010/283627
  17. Shelef LA. Antimicrobial effects of spices. J Food Saf 1983;6:29–44. DOI: 10.1111/j.1745-4565.1984.tb00477.x
  18. Chalmers NI, Oh K, Hughes CV, et al. Pulp and plaque microbiotas of children with severe early childhood caries. J Oral Microbiol 2015;7:25951. DOI: 10.3402/jom.v7.25951
  19. Munson MA, Banerjee A, Watson TF, et al. Molecular analysis of the microflora associated with dental caries. J Clin Microbiol 2004;42(7):3023–3029. DOI: 10.1128/JCM.42.7.3023-3029.2004
  20. Aghazadeh S, Haghgoo R, Mehran M, et al. Comparative evaluation of clinical and radiographic success of MTA and propolis in pulpotomy of primary molars. Iran Endod J 2018;13(4):508–514. DOI: 10.22037/iej.v13i4.21335
  21. Hill SD, Berry CW, Seale NS, et al. Comparison of antimicrobial and cytotoxic effects of glutaraldehyde and formocresol. Oral Surg Oral Med Oral Pathol 1991;71(1):89–95. DOI: 10.1016/0030-4220(91)90530-p
  22. Lewis BB, Chestner SB. Formaldehyde in dentistry: a review of mutagenic and carcinogenic potential. J Am Dent Assoc 1981;103(3):429–434. DOI: 10.14219/jada.archive.1981.0341
  23. Oosthuzen C, Arbach M, Meyer D, et al. Diallyl polysulfides from allium sativum as immunomodulators, hepatoprotectors and antimycobacterial agents. J Med Food 2017;20(7):685–690. DOI: 10.1089/jmf.2016.0137
  24. Farahpour MR, Hearaki S, Faraji D, et al. Hydroethanolic allium sativum extract accelerates excision wound healing: evidence for roles of mast-cell infiltration and intracytoplasmic carbohydrate ratio. Braz J Pharm Sci 2017;53(1):e15079. DOI: 10.1590/s2175-97902017000115079
  25. Elheeny AAH. Allium sativum extract as an irrigant in pulpectomy of primary molars: a 12-month short term evaluation. Clin Exp Dent Res 2019;5(4):420–426. DOI: 10.1002/cre2.197
  26. Houshmand B, Mahjour F, Dianat O. Antibacterial effect of different concentrations of garlic (Allium sativum) extract on dental plaque bacteria. Indian J Dent Res 2013;24(1):71–75. DOI: 10.4103/0970-9290.114957
  27. Mohammad SG, Baroudi K. Assessment of the potential of Allium sativum oil as a new medicament for non–vital pulpotomy of primary teeth. J Int Soc Prevent Communit Dent 2015;5:314–320. DOI: 10.4103/2231-0762.161762
  28. Asgary S, Kamrani FA, Taheri S. Evaluation of antimicrobial effect of MTA, calcium hydroxide, and CEM cement. Iran Endod J 2007;2(3):105–109. PMID: 24298290; PMCID: PMC3844763.
  29. Koruyucu M, Topcuoglu N, Tuna E B, et al. An assessment of antibacterial activity of three pulp capping materials on Enterococcus faecalis by a direct contact test: An in vitro study. Eur J Dent 2015;9(2):240–245. DOI: 10.4103/1305-7456.156837
  30. Zayed MM, Hassan RE, Riad MI. Evaluation of the antibacterial efficacy of different bioactive lining and pulp capping agents. Tanta Dent J 2015;12(2):132–139. DOI: 10.1016/j.tdj.2015.04.003
  31. Bakri IM, Douglas CW. Inhibitory effect of garlic extract on oral bacteria. Arch Oral Biol 2005;50(7):645–651. DOI: 10.1016/j.archoralbio.2004.12.002
  32. Ogunjobi AA, Abiala MA. Antimicrobial activity of senna and alata and phyllanthus amarus. Global J Pharmacol 2013;7(2):198–202. DOI: 10.5829/idosi.gjp.2013.7.2.74179
  33. Kshirsagar MM, Dodamani AS, Karibasappa GN, et al. Antibacterial activity of garlic extract on cariogenic bacteria: an in vitro study. Ayu 2018;39(3):165–168. DOI: 10.4103/ayu.AYU_193_16
  34. Fatemeh AM, Soraya H, Mohammad YA, et al. Antibacterial effect of eucalyptus globulus Labill) and garlic (Allium sativum) extracts on oral cariogenic bacteria. Journal of Microbiology Research and Reviews 2013;1(2):12–17. Corpus ID: 29279497.
  35. Mansour A, Maryam K, Neda R. In vitro comparison of the effects of garlic juice and chlorhexidine mouthwash on oral pathogens. Jundishapur J Microbiol 2012;5(2):398–400. DOI: 10.5812/jjm.2837
  36. Groppo FC, Ramacciato JC, Motta RH, et al. Antimicrobial activity of garlic against oral streptococci. Int J Dent Hyg 2007;5(2):109–115. DOI: 10.1111/j.1601-5037.2007.00230.x
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